Bonus mini-games: Successfully complete Story mode on any difficulty setting with any character to unlock ten mini-games.
Choir On The Green song in Free mode: Play EX Story mode on any difficulty setting with any character, then buy the Choir On The Green song in the shop in World 1 for 100 Coins to unlock it in Free mode.
Coin Collection mini-game: Successfully complete EX Story mode on any difficulty setting with any character to unlock the Coin Collection mini-game.
EX Story mode: Successfully complete Story mode on any difficulty setting with any character to unlock EX Story mode.
Extra songs in Free mode: Successfully complete Story mode on any difficulty setting with any character to unlock eighteen new songs in Free mode. Successfully complete EX Story mode on any difficulty setting with any character to unlock six new songs in Free mode.
Flight Of The S.S. Brass mini-game: Successfully complete EX Story mode on any difficulty setting with Mario to unlock the Flight Of The S.S. Brass mini-game.
Hard mode: Successfully complete Story mode on the Normal difficulty setting with any character to unlock the Hard difficulty setting.
Hop, Mario! song in Free mode: Play EX Story mode on any difficulty setting with any character, then buy the Hop, Mario! in the shop in World 2 for 200 Coins to unlock it in Free mode.
Note Pickup mini-game: Successfully complete EX Story mode on any difficulty setting with Luigi to unlock the Note Pickup mini-game.
Piroli song in Free mode: Play EX Story mode on any difficulty setting with any character, then buy the Piroli song in the shop in World 4 for 400 Coins to unlock it in Free mode.
Super Hard mode: Successfully complete Story mode on the Very Hard difficulty setting with any character to unlock the Super Hard difficulty setting.
Treasure Boxes mini-game: Successfully complete Story mode or EX Story mode on any difficulty setting with Mario to unlock the Treasure Boxes mini-game.
Very Hard mode: Successfully complete Story mode on the Hard difficulty setting with any character to unlock the Very Hard difficulty setting.
Whee! mini-game: Successfully complete Story mode or EX Story mode on any difficulty setting with Luigi to unlock the Whee! mini-game.
Where's The Exit? song in Free mode: Play EX Story mode on any difficulty setting with any character, then buy the Where's The Exit? song in the shop in World 3 for 300 Coins to unlock it in Free mode.
